Two more lives have been tragically cut short following a multi-vehicle crash on the A421.
It comes just weeks after a father and his three-year-old son were killed in an accident along the bypass on Christmas Day.
The deaths have sparked calls on social media for safety measures along the notorious bypass.
On Tuesday, four cars and two lorries were involved in a collision close to the A6 junction at around 2.10pm.
One person was pronounced dead at the crash site and another later died at Bedford Hospital. A further four people were injured including a woman who had to be cut free from her car and treated for serious chest injuries.
A spokesman for the East of England Ambulance Service said: “Three ambulance crews were dispatched along with an ambulance officer and two air ambulances.
“Despite the best efforts of all at the incident, two of the patients sadly died.”
A full collision investigation has been launched by Beds Police.
PC Andy Ralph from the forensic collision investigation unit is appealing for anyone who witnessed the incident to come forward. He is particularly keen to speak to those with dashboard cameras.
One man arrested on suspicion of causing death by dangerous driving has been bailed by police.
Anyone with information about the accident can contact police, in confidence on 101 or via Crimestoppers, anonymously, on 0800 555 111.
On Christmas Day Adesola Olusoga, 32, of Kempston, and his young son Isaiah Olusoga were killed following a single vehicle accident on the bypass between the Marsh Keys and Marston junctions.
Isaiah’s mother remains in hospital in a serious but stable condition.
A spokesman for Highways England, which is responsible for the road, said: “Our thoughts are with those affected by the recent incidents. However, due to ongoing police investigations it would be inappropriate to speculate on the causes of the incidents or to draw conclusions from them about the road’s overall safety record.”